FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583357
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT5451.5236373E-411.7816731
CTTCAAC8004.1043095E-610.7016871
TTCCTAA6457.588508E-49.9038619
CATTGCT6550.0091666628.7138411
CTCCAAA6550.0091666628.7138411
CTTGAAG9050.00175788187.8833791
AGAATTT11800.00329432646.61713033
CTTTCAA12400.00524572386.32895471
CTTTGAT14706.848622E-46.30938431
ACATTTT12550.00626655256.2216844
CTTTTTA15350.00115384736.04221151
Produced by FastQC (version 0.11.5)