FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687259
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAT5509.710238E-613.1922481
CTCTGTT6103.7077774E-410.7051861
CTCAAGA5600.002191223410.3653391
CAACCAT5600.002191223410.3653391
AATTACC6250.00559725949.2542046
CTTCGAT7954.10958E-49.1267131
CTTAAAT9552.7560123E-59.1171571
CTCAAAG7300.00192430928.9454291
CTTGATT9753.5177633E-58.9301381
CTTAATT7850.00371610168.318681
CTTCATG8600.0084327327.59321361
ATGAACT8600.0087063977.56611873
ATCATCC8700.0093495717.5059351
CCTTTAT9700.00294513077.4801411
CTTCATT14709.372945E-67.4038131
CTTCAAG9950.00377651897.29219771
CTTCCAG10000.00396572337.25573731
CCTGTTT10050.0041632667.2196391
CATCATT14103.3659968E-46.68968631
ATCAAAT16404.338821E-56.6363451
Produced by FastQC (version 0.11.5)