FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736891
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGAC4553.4048886E-412.9437691
CTTCAGT4050.002090471812.7240461
CACGAAT4400.00389048211.71190551
TACAGTA5600.002288873810.3112879
CTTCGAT7151.2219518E-410.2961811
CCCAGCT7953.5546484E-49.2600871
CTTCAAT12356.0939474E-68.34532451
TTTAATC7950.00435397638.1739812
CATTCAT10003.8576795E-48.0979451
CACAAAG8750.0086462097.57210541
CTTTCAT13551.7311373E-47.0629521
CACAAAA11950.00256790086.7765241
TGCATTT12750.0061968596.2288993
ATCAATA15200.0059824355.7002772
Produced by FastQC (version 0.11.5)