FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734801
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT9803.2250184E-58.9989391
CTCTCAT13652.3836159E-57.53757241
TCGAATG11050.00140322627.17741973
CTTATAG17501.6336016E-67.1391581
CCTTAAC10500.0056414016.99917461
CCCCGAT13651.942014E-46.99917461
CTTCGAA17551.2984376E-56.70006471
TTCGAAT14003.3448916E-46.6951642
CTCAAAT21609.551168E-76.46451571
CTATCGT15156.921606E-46.30618761
CTCACAA12900.0058329456.2667031
CCCCAGT14400.00269842296.12427761
CTTTATT49700.06.06266551
CCTCAAT23203.2463468E-66.0186871
CCCAAGA13550.0097055995.9660861
CTCAAGG18702.2255056E-45.89502671
CATTAAT20501.2512125E-45.7359091
CATAAAC27001.1872926E-65.7159931
AACCCTT15200.00609428345.69214063
CTTGCAT23252.1480297E-55.68965151
Produced by FastQC (version 0.11.5)