FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197074
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3954.4308763E-717.7532229
CACACGT3404.3194836E-516.5000537
ACACGTC4152.3603573E-413.5181168
GAGCACA5954.0366643E-410.6085064
AGCACAC6004.3608487E-410.52010255
GCACACG6050.00550509669.2739196
AGAGCAC7603.6827405E-49.2285453
ATTTTAG12156.6978676E-46.92681655
AATCCAA15854.3422842E-56.6372885
ATCAATT16756.287449E-45.86366222
CTTTAAT17158.41108E-45.731451
CAAGAAT16900.00439876225.400791
CTTTTAT23703.4236375E-45.036181