FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830196
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG3300.00893320413.1603461
CCCAGAA7151.4506097E-410.1233431
CCCAAAG7552.5105322E-49.58700751
CATAGAT6150.00485647389.4155321
CTTGAAT8558.6981786E-48.465721
CTTGTAA7850.00379992448.2985631
CTTAAAA9653.1555883E-48.2507881
CCCGCAT9000.00144524068.0424351
CTCAAAG8200.0056274087.9443551
CTTTTAT14751.2110304E-67.8515971
CCTTTCG8450.00736606547.70931531
CTTTAAT9400.0022176127.7002031
CTTCATT13552.6366426E-57.47857331
CGTTGAT9750.00317298277.4237851
CTTCCGT12104.4495222E-47.1783711
CTTCAAG10500.00652642876.8935151
CAACAAT10500.00652642876.8935151
TTGTAAA13702.4880766E-46.85759972
CTTTAAA13752.5521134E-46.843381
CTTCTTG11700.0024576376.8051361
Produced by FastQC (version 0.11.5)