FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767583
Sequences flagged as poor quality0
Sequence length100-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAT7700.00321765438.4538691
TAGGCAT8750.00114784128.233279
TCGATAC10306.697038E-47.69349243
CTCCAAA13552.6698848E-57.4729571
AAAGGGG11550.00225898026.8608633
GTTTGAA16053.372359E-56.75958351
CCCGCAT17802.0210928E-56.50135231
CCCGAAC14957.1443897E-46.2893521
CCAAAAA16403.2437043E-46.17430351
CTTGTCT13150.0083947456.05021331
CTTTGAT18802.970855E-45.77081541
GTCGGAA21454.8977538E-55.7322531
CCCGAAA19304.2393923E-45.62131171
CTTTTTT30404.3548062E-75.472151