FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479907
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA1200.005463248629.631739145
CTCAGAA3201.8594463E-518.197551
CTCCGAA4451.883326E-514.7216111
AACTACA3250.00822873713.3233816
GGTGTTT4100.009629813.009056145
CTTTGAG5157.441567E-512.7206171
CTTTTAT7602.5301703E-49.5776581
CTTTAAT7550.0025346648.6769771
CTCCTTT7950.004042358.2403991
CATAAAT8400.0066312197.79894921
CAAAGAT8400.0066312197.79894921
ATAAATA12707.9694914E-46.8197642
Produced by FastQC (version 0.11.5)