FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860951
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCT2700.002149145616.3082581
CTTCTAA4853.9319966E-513.6182351
CCCACTT4100.002347481212.5295151
CTCAAAT8757.235103E-710.90323451
CTTACAA8901.046905E-59.8948981
CTCCGTT8456.419029E-59.5533581
CTCAAGA6650.0082425148.828531
CCCAAAA9552.430037E-48.4529711
CTTTAAT12507.480772E-68.2193621
CTTTGAA14801.0350232E-67.93374731
CATCATT15051.1139582E-57.3143351
CTCAAAA11850.00243066796.812311
CTTGATT11950.00265530686.7553031
TCATTTC15351.4637157E-46.56423
CATTATT12300.0035945516.56307941
ATTTTGC19159.221512E-66.3891386
CTTTGAT15358.228097E-46.2151991
ATTTTGT19053.7897384E-45.6670686
AGATAAA18200.00130421095.53628976
TTTGATT20057.555859E-45.38410852
Produced by FastQC (version 0.11.5)