FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697502
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAG707.8161794E-414.498845105-109
CTCACGG4750.00778140410.6708811
CAGCATA8600.00903490657.53482774
CTTCATT12608.654737E-57.4708271
CTGTATT10400.00592353246.9624571
ATTTTGC12909.805646E-46.6976246
CTCCTTT14304.0973394E-46.58268641
AAGATAA14404.78309E-46.49948555
ATTTTGT13350.0014458516.4718626
CTTCAAA12700.0057700096.27169371
TTCATTT19005.551555E-56.06528662
AGATATT14650.0040996885.89756734
Produced by FastQC (version 0.11.5)