FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100780
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4102.4690962E-819.2315048
CACGTCT3804.884072E-616.9770939
CACACGT3707.377883E-515.4986087
AGCACAC7551.9419658E-611.3930165
AGAGCAC6950.00137531739.2824393
GCACACG6250.0060112079.1751766
GAGCACA8004.9418974E-48.9609464
GTCAGCT10306.884302E-47.6730371
GATGAAT17802.2249655E-56.45820471
ATATTGT18602.8982342E-45.78073076
Produced by FastQC (version 0.11.5)