FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556714
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2051.2247256E-831.586617
ACACGTC2452.1354936E-929.3662388
CACGTCT2508.4191925E-825.901029
AGGTCCA1650.00271609521.8041634
AGCACAC3106.7095425E-720.8879185
GAGCACA3101.5635025E-518.5687084
GCACACG3101.564713E-518.567046
AGAGCAC3202.054884E-517.9884363
ACGCGCC4850.00951874610.3841446
AAGAGCA7550.0027977598.5834362
AAAATCT11753.921827E-57.96083933
CTTTTAT9650.00287214957.49878031
AATTTCT10300.005735086.98517275
CAAAAAC10400.0059558926.9580031
ACGTCTG3550.0058844015.26937810-14
Produced by FastQC (version 0.11.5)