FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706284
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACT4300.003421623311.9146951
CCCAGAA6950.00113585389.4778761
CCACCGA6250.00506001349.3683541
CTTTGAT12356.5884396E-68.2968721
CGATGCC12305.4761185E-47.04899456
CCTTGAT10450.00559256837.00385331
TATTCTG11450.00199366346.9412597
CTTCAAT11950.0027296726.73717981
CCTCTTT12050.00297934846.6812691
ATCAGAA13900.00189452786.3185841
CAAAGAT12750.00537625236.31445461
CTTTCAT13300.0083329856.053331