FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173596
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACA5950.00377489289.7079941
CTCTTGG6300.00604687869.1686621
CTCCTGA10205.883672E-47.78662161
GTTGTTA9500.00260523157.57538842
CATGAAT11953.9713748E-47.25053261
CAACAAG15954.0734594E-67.2429561
GATTAAT10150.00481422767.11361741
CTTAAAG11400.0019200176.9669771
CACAAGA10400.0060971296.9426171
CTCAAAT12607.2779926E-46.8764971
CAACAAC18003.3479555E-66.81919241
TCAGAAC10600.00756866676.78926322
TATAGCA10700.0083315746.7220877
CAGAACT10900.0099515866.5993083
GTCTGTT12300.0042643836.4571981
CTCATAT12550.00526005266.3285691
CCCGAAA12600.00548234776.30345541
CTTTCAG20654.1910716E-66.29374271
CACAAAT13000.00758369556.1095031
CTTATAT15550.00117084426.0362821
Produced by FastQC (version 0.11.5)