FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6235485
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGTT22155.011316E-97.1918561
CTCAGAC20906.9997077E-76.58262871
CTCACAT22353.2314165E-76.47954461
CTCAAAT47850.06.3556421
CTAAGAT17158.3363426E-56.33314131
CTTAAAT43200.06.20168971
CTCTTAT36851.8189894E-116.09138771
CTCAAGA39653.6379788E-126.0264671
CCCACAT42650.05.9421151
CTCATAT48200.05.85881231
CTTGAAT54400.05.8566031
CTCAAAG66650.05.7579481
CCCAACT22751.9254569E-55.7290571
CCTTAAT31602.6668204E-85.7285531
CCCCCGT28005.093607E-75.6892721
CTCCTAT37209.094947E-105.6447811
CTCAGAA37259.422365E-105.63720371
CACTTAT30859.692485E-85.6331081
CTTAAAC25904.489253E-65.59142161
CTCAAAC46701.8189894E-125.5818441
Produced by FastQC (version 0.11.5)