FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293914
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT7903.0574847E-711.656929
CACACGT7351.6233716E-611.5654227
ACACGTC10408.886834E-47.4925038
GCACACG15251.6537005E-46.5043146
CAAACGC22602.4261615E-55.64641241
CTAAAAT19104.829078E-45.56757931
CTTTTAT34755.8707883E-85.30428741
CAAACTT21704.745298E-45.22411874
CAACTTT20350.00114184255.221499
Produced by FastQC (version 0.11.5)