FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782278
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCA4900.00995818910.3218839
CTTAAAG5800.002726395610.0963651
CCTCGAT8456.6003005E-59.5288181
GTTTAAT6200.0047324119.4449861
CTTTAAT11352.1062697E-69.0289081
ACGGAAT7550.0027099028.61461453
CTTGTAT7700.00288360578.5556851
CTTCAAA14507.8897756E-78.0770921
CTTCTGG11452.0703644E-47.67147351
CTCCTGC12708.3500265E-57.4927751
CTTCCAT15802.8753311E-67.41252141
CTTTTCG11350.00158414587.0941421
ATCAAAA18353.643543E-66.7813461
TTCAAGA14153.707354E-46.63725232
CATTGAT13300.00115000516.6043891
CTTTTCT21106.78765E-76.5913471
CTCCTTT14150.00231276226.2076591
CTCTTCT13250.008007686.0768691
CATCAAT15850.00124772716.0036741
CAACAAA15200.0051413175.77884051
Produced by FastQC (version 0.11.5)