FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553266
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG3850.001523196913.2698561
CACAAGA5150.001036941311.3373521
CTTCGAT6204.0786265E-410.5944811
CATCGAC5700.002416233510.2433981
CCCAAAA5800.002791298810.0667871
CTTATAT8053.999002E-59.9729981
TTCGATT6200.00538451279.2972922
CTTGAAG7200.00160794099.1230261
CTATAAT6700.0091592868.7145321
CCTTCAT8507.547041E-48.5863771
ACATTAT8150.00551548047.9612024
TATGCAG8150.00551996437.96048269
CAAGAAA12004.2705615E-57.9066221
CTTCCAT9350.00193715567.80579761
TCAAGAA10600.00746526436.79754732
CAAAGAA16002.0870575E-46.38611751
CTTTCTT14950.00441147065.8582641
AAAAGAT17750.0057206245.2800593
Produced by FastQC (version 0.11.5)