FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695207
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6003.7471182E-1016.7923097
CACGTCT6602.3754183E-814.1753269
ACACGTC8103.4422374E-711.5502648
AGCACAC10206.6204466E-69.172275
GCACACG11603.5686153E-68.6856786
AGAGCAC13001.6410611E-57.75166853
GAGCACA13151.5835412E-47.11587144
GTTCGAT12600.00539509356.3135631
CTCCTGA17155.769841E-45.9035911
CACAGAA16850.0029964895.57950741
TGAATCT18300.00139874625.50664953
GAAGAGC22851.2767462E-45.38040641
GGTAAAA21550.00185532035.0338051