FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644452
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT4351.715035E-514.8698611
CTCAGTT3000.00497949514.3741991
CTCATTT5601.8323967E-411.5506951
GATCGAG4650.007173556410.788235
CTTTAAT6355.8611E-410.18643951
CTTTGAG6709.5921685E-49.6543131
CAACAAT7653.0739745E-49.39491
CTTCAAT8751.1786449E-49.0352111
GTCAAGG6400.0071402798.9838741
CTTTACA6600.0091878918.7116361
CTCCAAA7600.00302201328.5110391
AACAATT8100.0055084157.96274142
CTTTGAT10105.552506E-47.82753371
TTTCATC16804.95963E-45.97205648
CTTCTGC14800.00468201385.82737731