FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466336
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5800.024.7519917
ACACGTC6850.020.9578918
CACGTCT6850.020.9578919
AGCACAC9300.016.9803985
AGAGCAC10700.014.7586643
GCACACG10100.014.2140146
GAGCACA11750.013.4398054
AAGAGCA22751.0020813E-86.9457012
GAAGAGC19752.814577E-76.936241
CTCCCAT11950.0032011676.6368561
ATATACC13750.00209130726.2645046
TTGATTC12650.00607484536.2418063
CCCAGAA14150.00274462746.11452051
TCAAACT14750.00455248035.84337852
CCCAGAT16050.00174095855.8399071
GTTTGAT16150.00187496155.80374671
CTGAAAT19354.578495E-45.58917761
GTTGAAA17600.0051900275.32559731
CAAAGGT18150.0074425435.1642151
Produced by FastQC (version 0.11.5)