FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801731
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAC4700.00797278610.6363171
GATGTAG6900.0013957599.2668125
TAACATT6500.00892125358.7435249
CTCAAAG7700.00360422748.34723951
ATACCGT7750.0040065018.2499388
CTTCAAG9904.8007251E-47.93502951
CCCGGAA11754.3156637E-57.90126371
CTTTAAT10357.725713E-47.5900281
CAACAAT10650.0085235976.70565941
CCTTCAT12000.00369096156.54639961
CTTTTAC12850.00752701336.11336951
CTTTCAA15650.0093183325.4759311
Produced by FastQC (version 0.11.5)