FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141955
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACT5200.001042361811.3322311
CCCCTGT6300.00513028179.3535881
CTCCGAA11402.008559E-58.3997681
CTCTTAT9953.6374904E-48.1432611
CCCAGAT10255.0052366E-47.90492251
CTTTAAT17953.6063284E-87.79682731
ATCGTCT10506.4792455E-47.716711
CAACAGA14405.6726476E-67.6728651
ACAGCTC10507.957749E-47.56969642
CTTCAAC14657.238059E-67.54192831
CAATCAT15902.8827653E-67.41227671
CTCCTGA12003.3120002E-47.365951
TCCCGGC14657.5623386E-56.90502832
CATCACG10900.0079159456.75775241
TCGATTT12200.00388733766.51461653
CTCCTGT12850.00546353736.3054831
CTTGATT14250.00233538736.20290571
CTTCTAT14400.00262636276.1382921
CTTCGAT13250.00751107656.11512851
CTTCTGG13450.0087704016.0241971
Produced by FastQC (version 0.11.5)