FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664109
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8100.027.0504727
CACGTCT9800.023.0792739
ACACGTC9550.022.9433358
GAGCACA14500.016.0906874
AGCACAC14750.015.8176665
AGAGCAC15300.015.2493433
GCACACG17050.013.26874356
TTCCCTA10901.4460966E-911.0235288
TCCCTAC9202.2394725E-69.987439
ACACTCT10157.301678E-79.7625621
CCTACGT6350.00769192178.9046018
AAGAGCA32550.07.81952052
GAAGAGC30100.07.7597771
CCCACAT16751.481636E-67.1834971
CACTCTT15051.8877397E-57.04666142
CACGAAA19952.2537668E-56.03125671
CCCACAG15950.00201704775.76878641
CGATTGA16050.0022013345.72660164
TCCGATC17750.00119807615.5747828
CCAACAT20652.3812447E-45.4840541
Produced by FastQC (version 0.11.5)