FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697375
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6450.00731505368.9589757
CTCTAAA7600.00279962398.5848641
CTTCACT12901.4860725E-68.4295821
TCGAGAT10600.0073079186.8143143
CTCGTTT12300.0040914196.48323871
CCCAGAA13050.00757387236.1106391
AACTTGA14200.00279834946.10408976
CAAACGG20802.9560555E-55.9250231
CTTCAAG22351.417057E-55.8384751
GTGAAGA22401.557289E-55.80433567
CCCGATA15000.0049507895.7995521
AAGATCA20551.6593593E-45.62388135
CCCGGAA24004.426771E-55.43707941
CTTCGAT18900.00192058065.36995551
CTTCGGG19050.0021212975.32767251
CAAAGAA42951.0659278E-95.23242431
CAATGAT25058.714603E-55.2091781
CTCAAAG18100.00676357655.206781