FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302778
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAT7351.4577345E-611.6681441
TAGATCT4959.087207E-411.5196953
CTTACAC4800.009274607510.4223271
CCCGTCC7552.4946346E-510.4124671
TCCCAAC7400.00255325448.6722882
CCTTATT12851.7230286E-68.3424961
CCCGCAA11252.2306066E-47.6231881
CTTTGGT12408.3685125E-57.4925481
CCCCGCC11750.00294098446.69056371
CTGCCCA15001.1894792E-46.6702891
CTTTTAT24605.2210453E-86.3913921
AATTTCG12350.005086266.34914545
GTTCTCT12450.0053854716.31438731
GATCTTG15059.222267E-46.15738635
CTTTCAC14050.00279751426.10397621
GTTTCAT18202.2468582E-45.8901691
TCAAATC16300.00238809765.686942
CCTTCAT16650.00299145125.5800361
GGATGAT18050.00128567925.54317671
CCTGATC15650.0092559975.47992751
Produced by FastQC (version 0.11.5)