FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781450
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT4402.727169E-413.2882891
CAGGGAT4350.00377137611.7608991
CCCAGTT4800.00785110210.6583151
CTTAAAC4850.00847842510.5484341
CCTGTTT9853.4809527E-69.6458141
CCCATTC6950.00115111039.464321
GTCATAA6250.00512107149.3549551
CAATCTC7000.00137275619.2836434
CTTTTGT14851.3782483E-88.8588581
GTTTCGA6700.0090628798.7266371
GCCTTCA8306.633406E-48.6995314
CTTTAAA11502.4690224E-58.2618491
GTTCCAT9150.00154606927.9874961
CTTCAAT11903.787972E-57.98413941
CCTTCAT11151.5499476E-47.8657131
CTTTCCC9300.00181474467.85866451
CCAATAT10100.00458256437.14911943
CTTTCAT13551.8931649E-47.0119011
CTTCCGG13500.00138564426.4964961
CTTTTAT17054.6641592E-46.0011621
Produced by FastQC (version 0.11.5)