FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610350
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATCT4359.0448157E-716.5801893
CTTTGAA10255.3774784E-810.5920411
CTTACAA5550.00207527510.43300151
AGATCTT4850.00934924110.4096244
CTCTTGT5800.00299157959.9833031
CTCCAAT5850.0032119899.8979761
CTTCAAT10559.071282E-79.6047891
CTCAAAG6200.0051899049.339221
CTTTAAA9101.671655E-48.7491041
CTTGAAT6650.0092246998.7072421
CTTCATT10851.3403995E-58.67213251
CTTTGAT11352.362553E-58.2900991
CTTCAAA13101.8857736E-68.2876661
CTTTCCT9703.3339814E-48.2079221
CTTTTAT11251.921513E-47.7204211
CTCCTTG8550.008180057.61883641
CAGATTT9600.00282074287.5128983
ATCAATT8650.0093658297.50421242
TTCAATA11500.00212577826.89880042
CATCAAT12850.0065441666.19586371
Produced by FastQC (version 0.11.5)