FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061717
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG5700.002816211410.0584437
CCCCGCT6200.00539585259.2971071
CTCAACC14909.80874E-56.7700411
TAACCGC10750.0090312626.6666425
CTCTGAT18505.3179483E-66.62104751
GTCTCCG12650.00584355286.2654421
CTTGGAT14050.0025553216.1539571
ACATAAG14050.00271392266.1209746
GTCCGGA14150.00276662016.11046651
CGAACAT16150.00189105855.7998981
CTTCGAT24158.94229E-65.6687331
CTTGAAA38654.5656634E-105.59269671
CCCAAAA17050.00360050845.4937451
CTCATAT21403.068623E-45.3871091
CTTGATT27101.080187E-55.31753351
CTTGAAT21703.7395977E-45.31263261
CTTTAAA30002.0977131E-65.28385641
CTTAAAG19450.00297253955.1863041
CGTTCAT23702.3329772E-45.16832831
CTTCAGG25401.1938457E-45.1060881