FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524912
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC3905.813594E-616.669995
TACGCTA4151.8360032E-413.9251334
CTCTAAT6353.9253755E-511.5152031
CGCTACT5059.6088677E-411.4434266
CTACTGA5250.001329653211.0074868
CTCAGTT6150.0044695279.51174451
CTCATAT11602.833549E-79.4553711
CTCCAAG11101.5834794E-69.2225371
AATCTAC7050.00146088349.2216985
CTCCATT15302.3756002E-99.0804531
CTCTCGC7750.0030903428.4915331
CCCACTT12155.374557E-68.4255271
CCCAGTT10457.259539E-58.3967311
CCCTCAT8800.00104780278.3092661
CTCATGG11552.5969084E-58.230131
CTTAATT10751.009931E-48.1624051
CTCAGGA8100.00460377068.1246151
CCCAAAT13552.6530288E-68.0946361
CTCATTA9200.00162573837.94799331
CTCCTCT11101.4656624E-47.90503171
Produced by FastQC (version 0.11.5)