FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991681
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAGG4450.005094290211.2970678
GTTACAA8750.00113170458.2442461
CCCGTAT8200.00580315947.9174921
TGAGCAA8200.0060394867.88236148
AAGGTGC8550.0087756167.5600735
CTTGAAT11703.146174E-47.39868161
GGATAAG11300.00176570047.02221
CTAAAAT10450.0064419836.9030761
CGAAGAA16100.00179398685.82473851
TTGATGT15200.0063508955.6700553
CAAAGAT18250.00131233985.53380871
CGAAAAA17050.0035450825.5001931
CTATTAT15800.009271415.47877071
CTTTTGA15850.00959894255.46148731
CTTTCTT30151.24803555E-55.0244781