FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803464
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT6607.0659225E-510.8697419
ACACGTC6100.00489089829.4085648
CACAGGG8100.0052074848.0142511
CTACTGT9750.00328826727.397771
AACTTGG10050.00465868137.13813926
CCCACAT10800.0088759926.6785421
CACAATT13100.0011469776.6071681
CCCGAAC12150.00379476256.530131
ATTCGCT13300.0013615346.50781251
CTTGAAT18854.8661044E-56.12229161
CTCAGAA13050.00798213956.07977631
CCACAAG13200.0089833546.0106881
CGGGAGA15250.0062895525.6756661
CATTGAT30153.901514E-75.50232171
TTCGCTG18850.0021005955.33188722
CTTTCAT35305.6621502E-85.31256251
CTTCGAT17700.0055241545.29755541
AATTTCC28655.4907377E-65.2583055
CTTTGAT31559.639716E-75.2581611
TTGGTGA19300.00284820545.20395958
Produced by FastQC (version 0.11.5)