FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098973
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT6901.1622993E-410.3483341
CTTTGAA18200.09.8081741
GAAACTA5850.00372869179.7223754
CTTTCAT18151.8189894E-129.4417871
CCCAAGG6300.00662583959.0671131
CTTATTT12056.9244015E-78.8884041
CCCTGAT8408.357375E-48.5004181
CCCTTCT10257.6411045E-58.3594351
CACCCAT7950.0048171638.0834161
CAATGAT15152.605084E-78.0122751
TCGCTTT11603.3708697E-47.3546413
TCCGATT9850.00415468737.2220252
CCCTTTT10900.00134376297.2058591
CTTTATT14256.143542E-57.01508141
CTCAAAG11350.00206129076.92016361
CTTTTAC13452.3034144E-46.90145441
CTATTAT10450.0071134446.8328721
CTTTGTT17051.2933195E-56.70062261
CTTCGCT10700.0089378826.67322541
TTCGCTT11900.00352541476.57568362
Produced by FastQC (version 0.11.5)