FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947743
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT8153.6289384E-711.5035239
CACACGT8104.8972157E-59.7938367
ACACGTC9552.938811E-48.3068138
GCACACG11500.0021334626.89826636
CCCACAA20002.319155E-66.52649641
GTTCTAC12450.004629446.4070911
AGCACAC12750.00628166836.2219665
CAAACAT19354.238964E-45.62144371
CCCCCCC5357.465129E-65.4319835120-124
AATTATG17700.00553391275.29682067
CGATTTT27751.6028473E-55.1982454
CAAACGG18550.0089782355.0820281
Produced by FastQC (version 0.11.5)