FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123648
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAT1800.004187400520.2374041
CCCGAAC7200.00163696319.1068321
CCCAGCT11151.7449509E-58.4942651
CTTTGAG10406.578115E-47.70578051
CGCTCCA10150.00483510557.11039974
CTCAAAA14807.756359E-56.8916561
CTATGAT10900.0088012996.68391281
AATTTAC11900.00303221566.67093135
AACGATT11950.00316708936.64331442
CTTGTAT12400.0042247226.46291261
CATCAGG13750.00176839456.3582241
CTTGTCC12600.00551031866.3003235
CCCGGAT12750.0056469266.28549961
GAATTTA15108.2655717E-46.2133594
CTTCAAC12900.00637682676.21241241
CCTCCAT13050.00718942246.14100461
CCTTTAT16653.585352E-46.12591651
CTTTAAT15500.00101093336.11038971
TTCAATC14450.0034306175.99340252
CTTCAAG14600.00346489465.98805331
Produced by FastQC (version 0.11.5)