FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670177
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAA5550.002031447610.4600911
CTTCAAA11452.4056135E-810.1403511
CTTGTAT7351.8662444E-49.8730461
CCCAGCT6300.0057966639.2148431
CTTCTCT8801.12935275E-49.0708611
ACCGGAG6650.0093023298.6985616
GTCATAT7700.00311774278.4818431
CTTCAAT9753.428074E-48.1870331
CTTTCAA11402.1743175E-47.6386191
CTTCATT10557.9842185E-47.56621551
CACAAAA11000.00124536267.25668861
ATCAGAA12750.00587570066.26067261
CTTTCTT16804.2245098E-46.04724031
CTTTTTT27201.0101729E-55.33580061
CTTCTTT21300.00151361125.1103441
Produced by FastQC (version 0.11.5)