FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872295
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCTT6605.786719E-511.08377551
CCCACGG4700.006669902210.8951161
CTCCAAT8254.4041353E-610.6404261
CTCAAAT7953.404233E-510.1217871
CCTCGAT7301.6075849E-410.0209471
CTTAAAT6350.0057929519.2161161
CTCATAT7200.0015763939.1441151
CTTCAAT14256.123464E-78.2136611
CTTCTGA9300.00179888687.86590531
CTCATTT9400.0019985127.78222561
CTCTTTT19257.766319E-66.46025751
CTTCCAT16202.3853332E-46.32185751
CTTTTAT18904.1059375E-56.1928391
CTTTTTT32258.3309715E-106.12443071
AATAACA13050.00781304956.09154037
AGGAATA14750.00424344335.87975364
CAATAAT15800.0079458675.55591771
CTTCTTT32001.6640479E-75.4864691
CTTCCGG17800.0049914885.3426291
AAGGAAT17750.00556809135.2931583
Produced by FastQC (version 0.11.5)