FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097504
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4950.034.1362157
CACGTCT5000.033.7948539
ACACGTC5150.032.810548
AGCACAC6950.025.3304985
GCACACG7250.024.2779126
GAGCACA8050.023.6208744
AGAGCAC7850.021.5312713
AAGAGCA15050.012.6419112
GAAGAGC14250.011.9137481
TAGGATC3651.4679244E-98.12282230-34
CCTCAAT9603.8244235E-48.1053821
CAAACGC9000.00189457467.82502173
CAGTCAC5551.8189894E-127.379996320-24
GTTTCAT15352.4644194E-56.9124971
CCAAAAC11750.00327402176.6222691
ATATTCG12300.00554525956.29646646
CTCGTAT5901.5788828E-96.222619535-39
AGAAGTT14150.00356708585.97245454
CAATAGG6902.582965E-105.93375230-34
CCAGTCA8803.6379788E-125.617414520-24
Produced by FastQC (version 0.11.5)