FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501231
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAG4900.00983713710.3399481
AACTCGG8750.00114742858.2333876
CATTACT8450.007375087.70907351
CCCAAAT9500.00246389827.61890841
AATTCGC10050.0044699227.1683715
CTCCAAG10450.00624035356.9262811
CTTTGAC16253.964958E-56.6811971
TCCGATT15151.21888115E-46.6580242
ATTTAAC12650.00584985736.2645336
AAGATTG16353.2803722E-46.16874555
CAATGGA16553.61244E-46.12274841
CGATTCC15650.00129860245.98472934
CCACCGT14550.00358955635.9694551
TCAACGC14750.0043974525.8616413
TTCAACG16450.00235453555.6938682
CTTTAAA21806.191891E-55.64428331
CATCAAC26556.963577E-65.45232631
Produced by FastQC (version 0.11.5)