FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872878
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT6053.3746728E-612.4648981
CTCCGAG5803.0018124E-511.8201631
CTCGGAT4400.00661713210.9067861
CTGTAAT8007.706608E-48.5696181
CCCAGCT7450.00386924748.2820461
CAATGAT10151.0943852E-48.1052541
AAGGATT12151.3021385E-57.88598544
CTTTTAA8850.0020909197.7465471
AATCAAG10008.4313366E-47.52829936
CTTTGAT11200.00274830686.73327061
AAAGATT14601.4683076E-46.5626522
CAAAAAT15352.7498382E-46.25275041
CGAAGAA15503.1177234E-46.19224071
TCAATAT14750.0077593515.56792553
Produced by FastQC (version 0.11.5)