FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635082
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTT4057.4222207E-616.2438181
CGTAGCA3150.00615125113.9232721
CTAAAAT4400.004101255411.6290961
CTTAAAT6504.9870025E-511.245721
CTCCGAA4650.00619182611.0038771
AACTCAA5300.001434721410.9058365
CTTCTCC10901.25431725E-58.7180121
CTTGCAG9800.00302740547.4588961
TATTATG10300.005507777.01467567
CTTTTCC19050.00190467555.3719711
AAAGAAT18550.0093396855.06342363
CTTTTTC26101.4496433E-45.0411851
Produced by FastQC (version 0.11.5)