FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820804
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4957.360389E-411.8118341
CCCCTGT4650.006201699411.00215151
CTTAAAT9001.3355729E-48.9326991
CTTCGGT8350.0060705977.87750241
CTCTCTT8350.0060705977.87750241
CTCAAAA11201.632743E-47.83061271
AGGATTT12405.99272E-46.9939663
CTTCATT13601.9811641E-46.98613551
CTTCAGC10500.0059419216.9605451
CTTTAAA12807.5743475E-46.8517861
CTTTTAA13109.85722E-46.6948751
GCCATTT12850.00665314366.18662173
CTTTGCA15850.00127132075.9944131
CTTTATT16500.00205575125.7582691
CTTTTCT20156.565946E-45.4406241
CTTTTAT18600.0084394735.1081421
CTGCAAA18650.0087076455.0944471
Produced by FastQC (version 0.11.5)