FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134333
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5151.8189894E-1220.9728189
CACACGT5505.456968E-1219.6381867
ACACGTC7001.0913936E-1116.458678
AGAGCAC7901.2259989E-913.6715523
GAGCACA9158.112693E-1012.59244254
TCAGTCG4600.006394634510.9575389
AGCACAC9301.7322418E-710.8392375
CTTACGA4750.00798646510.6344841
GCACACG11553.117202E-89.9745116
AAGAGCA12701.3389945E-79.0749092
GAAGAGC13101.9753898E-68.262891
CTTTTAT13600.00174031786.36728571
CGAGAAC14150.00271674226.1197941
CGGGAAA15100.00560304765.73477361
AGTCACG4300.00191077945.023234420-24
Produced by FastQC (version 0.11.5)