FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658438
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT4350.00415312911.6096794
CTCAGCC4500.00487084411.364281
CCCATTT5802.1808645E-411.3362881
CTCAAGA4650.00621818510.9976891
CTTATAG6153.752512E-410.69113351
CCCTTTT6550.0075508658.922881
CTTCCGG9251.804737E-48.687751
CTTCAAT12457.521505E-68.2151411
CTTGAAG9550.00236346047.6498521
CTTCGAT8800.0097379947.47164441
CTTCCTT13601.9889693E-46.9833021
TAGATTT12808.768938E-46.7636753
TTCAATC10850.0092333196.64989382
CTTTTGA13950.00201318676.2843941
CTTTCAA14000.002095826.261951
CTTTTAT18301.7878131E-45.9882041
CTTCATT15350.00585379455.7112251
CTTTATT16900.00274376975.6196981
CTTTTTC26553.4322948E-55.2281191
Produced by FastQC (version 0.11.5)