FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487073
Sequences flagged as poor quality0
Sequence length100-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4850.033.275877
CACGTCT6500.024.8289179
ACACGTC8650.018.6575688
AGCACAC9100.018.5041585
GCACACG11500.014.6424216
AGAGCAC11800.014.2711163
GAGCACA12000.013.4499024
AAGAGCA17550.09.9968832
AGTCACA4901.3969839E-96.87320420-24
GAAGAGC24851.27893145E-86.50915241
TCAAGGG12800.0086863066.0301217
CAGTCAC6451.3902536E-85.65662920-24
TCACAGT6855.365655E-85.32656625-29
TTGGCTA6556.429909E-75.142063630-34
CCTCGAT23402.7663208E-45.1092371
CCAGTCA6903.4963887E-75.084344420-24
CTTCAGG18100.0096340255.05112081
Produced by FastQC (version 0.11.5)