FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208615
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT3250.00829892513.3085851
TTACCCC4350.004267712611.5687229
CCCCAAT5008.99164E-411.5341071
GGTAGAT7650.00312442918.4809611
CCCACGA8200.0058405357.91211651
TTCGATT9900.0039327117.26295332
AGTTTCA11650.00252268116.7888665
CTTTCAT20252.133557E-56.05184651
CTATTAT15700.00133929885.96908471
CTTCAAC16100.00180965735.8207841
CATCAAT18702.8873488E-45.7824741
CTTCCGT16350.00217460845.7317811
CAACAAA18100.007217595.17760371
CAAAGAA27206.4059124E-55.03557061
Produced by FastQC (version 0.11.5)