FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775366
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6500.036.4130257
ACACGTC8100.027.4486268
CACGTCT9600.023.9068689
AGCACAC11300.020.9461355
GCACACG11050.020.7703536
AGAGCAC13000.017.1059783
GAGCACA13900.015.9983974
AAGAGCA24800.09.8382052
GAAGAGC23100.09.3562611
CTCGTAT7500.07.86151235-39
TCGTATG8600.07.188459440-44
ACCAAAT11700.0025860236.7732941
TCTCGTA8650.05.985079335-39
ATCCAAA16804.926658E-45.97688536
ATCTCGT11100.05.830060535-39
AGTCACC9950.05.78097120-24
CTTTGAA20550.00103831585.2586291
TATCTCG10401.8189894E-125.25452935-39
GTTTCAC17950.00660158275.2176141
CGAAAAT23502.05889E-45.2116371
Produced by FastQC (version 0.11.5)