FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188609
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5509.007936E-613.290521
CTACGAA5751.4237798E-512.7126711
CCCAAAT9401.303124E-811.6645521
CTCAGAA7401.5571231E-510.8658971
CTCGAAA8709.382016E-48.4020531
CATCAAT22801.1823431E-107.6945121
CTCAAAA17201.6590093E-77.64977551
CTTCTAT16053.72077E-67.28701351
GTATTCG13101.4600762E-47.1624266
CCAAAGG13451.727467E-47.065221
CCCAAAA13650.00156882826.42618561
CTTTACA13050.0069460096.16150571
CCTGCAC14400.00286171686.0914881
CCTGAAT13250.0081312366.06850151
GTTGGAA13300.0084542526.0456871
CTCCATT14550.00321328246.02868941
CTTTCAT23302.4158535E-55.64704471
CTTTGAT20801.6614022E-45.6229121
CTTTTGG15650.00721819055.60494761
CTTTAAA17850.00520963185.3236531
Produced by FastQC (version 0.11.5)