FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472912
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAG20152.8019767E-66.45215651
CCCACAT23701.434928E-76.39997671
CTCCGAT27156.8212103E-96.3848231
CTCCAAG22652.9000876E-66.0588871
CCTCGAT31604.5238266E-95.94283531
CCCGTAT27203.0456977E-75.84199521
TCTAATA17608.6590333E-45.7192932
CCCTGTT32605.5377313E-85.5389811
CTCGAAG23603.5988327E-55.5089391
CTCTATT23804.1160147E-55.4626451
CTTCGAG25452.0693167E-55.392291
CCCCAGT17500.0047594695.3655321
CTAAAAA39253.6816346E-95.3366141
CTCACGG21803.859549E-45.30116031
CCCAAAG26353.6865677E-55.2081131
CCCGCGA18050.0068423835.20203831
CTTCAAA64100.05.1833111
GTTTAAT25159.848281E-55.16942171
CCCAAAA36351.0572876E-75.1662611
CTTATTT50752.7284841E-115.12358431
Produced by FastQC (version 0.11.5)