FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503941
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT1750.003848791620.5315883
CTCCCGG3100.005788556814.0507331
GAGATTT5158.394521E-512.5581563
CCCAGTT4300.003634479111.8178631
CTCTGAA4500.005103192311.2926251
CTTCGAA4650.006514082710.9283471
CTTCGAG5650.002346994410.2790021
CTTTTTT15550.00723431175.6022211
CTTCTTT15850.0089295375.49618531